Purification and Reversible Inactivation of the Isocitrate Dehydrogenase from an Obligate Halophile
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چکیده
منابع مشابه
Purification and reversible inactivation of the isocitrate dehydrogenase from an obligate halophile.
The nicotinamide adenine dinucleotide phosphate-specific isocitrate dehydrogenase of Halobacterium cutirubrum is rapidly inactivated at low NaCl levels. As much as 75% of the initial activity can be restored by dialyzing the inactive enzyme against 4 m NaCl. A mixture of 4 mm isocitrate and 10 mm MnCl(2) gives the same protection as 4 m NaCl but does not replace the NaCl requirement for reactiv...
متن کاملNature of the inactivation of the isocitrate dehydrogenase from an obligate halophile.
The nicotinamide adenine dinucleotide phosphate-specific isocitrate dehydrogenase (ICDH) of Halobacterium cutirubrum is rapidly inactivated at low NaCl levels. From sucrose gradient analysis, it was estimated that the active ICDH has an S(20,w) of 5.3 and a molecular weight of 75,000. The inactivation by removal of NaCl causes an unfolding of the protein yielding a less-compact conformer with a...
متن کاملReversible inactivation of isocitrate dehydrogenase in Escherichia coli.
Escherichia coli can grow aerobically in defined media containing inorganic salts and any one of a wide range of carbon compounds. Whatever the nature of the carbon source. it is fed into the amphibolic pathways [glycolysis, pentose phosphate pathway, Krebs (tricarboxylic acid) cyclel, from which stem all the biosynthetic pathways required for making the components of new cell material. The ope...
متن کاملPurification and characterization of NAD-isocitrate dehydrogenase from chlamydomonas reinhardtii
NAD-isocitrate dehydrogenase (NAD-IDH) from the eukaryotic microalga Chlamydomonas reinhardtii was purified to electrophoretic homogeneity by successive chromatography steps on Phenyl-Sepharose, Blue-Sepharose, diethylaminoethyl-Sephacel, and Sephacryl S-300 (all Pharmacia Biotech). The 320-kD enzyme was found to be an octamer composed of 45-kD subunits. The presence of isocitrate plus Mn2+ pro...
متن کاملCloning, expression, and purification of choline dehydrogenase from the moderate halophile Halomonas elongata.
Choline dehydrogenase (EC 1.1.99.1) catalyzes the four-electron oxidation of choline to glycine-betaine via a betaine-aldehyde intermediate. Such a reaction is of considerable interest for biotechnological applications in that transgenic plants engineered with bacterial glycine-betaine-synthesizing enzymes have been shown to have enhanced tolerance towards various environmental stresses, such a...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1969
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.99.1.161-168.1969